The pseudotyped alpha-Nipah Virus particles are created by combining the structural proteins of the Nipah virus, including Fusion (F), Glycoprotein (G), Matrix (M), and Nucleocapsid (N), with an alphaviral vector to allow for the expression of a reporter gene GFP. The pseudotyped alpha-Nipah Viruses are single-cycle viruses with self-replicating RNA for rapid quantification of neutralizing antibodies and entry-inhibiting drugs. These pseudoviruses are BSL-2 safe and ready to use for studying viral entry.